prox 1 Search Results


94
Novus Biologicals prox1
Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among <t>Prox1-neurons</t> (E) .
Prox1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals mouse anti prox1
Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among <t>Prox1-neurons</t> (E) .
Mouse Anti Prox1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human prox1 affinity purified polyclonal ab goat immunoglobulin ig g
Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among <t>Prox1-neurons</t> (E) .
Human Prox1 Affinity Purified Polyclonal Ab Goat Immunoglobulin Ig G, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems prox1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems goat anti hprox1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Goat Anti Hprox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals prox1 ap
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Prox1 Ap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems goat anti human prox1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Goat Anti Human Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene human prox1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Human Prox1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Proteintech prox 1 protein
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Prox 1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene prox 1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Prox 1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene prox1 cdna
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Prox1 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Novus Biologicals rabbit anti prox1
Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker <t>Prox1</t> and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Rabbit Anti Prox1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among Prox1-neurons (E) .

Journal: bioRxiv

Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy

doi: 10.64898/2026.04.06.716727

Figure Lengend Snippet: Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among Prox1-neurons (E) .

Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X), PROX1 (Novus Biologicals, #NBP1-30045AF647), OLIG2 (Novus Biologicals, # NBP2-89201AF594), and PAX6 (Novus Biologicals, #NBP2-34705AF647) at 1:100 followed by incubation for 1 hour at 4°C with agitation.

Techniques: Variant Assay, Amplification, Sequencing, Fluorescence

Sorting scheme to enrich astrocytes, oligodendrocytes, Prox1-negative neurons and Prox1-positive neurons for amplicon sequencing.

Journal: bioRxiv

Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy

doi: 10.64898/2026.04.06.716727

Figure Lengend Snippet: Sorting scheme to enrich astrocytes, oligodendrocytes, Prox1-negative neurons and Prox1-positive neurons for amplicon sequencing.

Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X), PROX1 (Novus Biologicals, #NBP1-30045AF647), OLIG2 (Novus Biologicals, # NBP2-89201AF594), and PAX6 (Novus Biologicals, #NBP2-34705AF647) at 1:100 followed by incubation for 1 hour at 4°C with agitation.

Techniques: Amplification, Sequencing

Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker Prox1 and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate

Journal: Angiogenesis

Article Title: A xenograft model for venous malformation

doi: 10.1007/s10456-018-9624-7

Figure Lengend Snippet: Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker Prox1 and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate

Article Snippet: Primary antibody incubation with anti-CD31 (1:50, Dako), vonWillebrand Factor (vWF) (1:100, Dako), αSMA (1:500, Sigma), VE-Cadherin (1:50, Santa Cruz), and PROX1 (1:50, R&D Systems) was performed for 1 h at room temperature (RT).

Techniques: Marker, Expressing, Staining, Positive Control, Isolation, Immunofluorescence, Quantitative RT-PCR