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Image Search Results
Journal: bioRxiv
Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy
doi: 10.64898/2026.04.06.716727
Figure Lengend Snippet: Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among Prox1-neurons (E) .
Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X),
Techniques: Variant Assay, Amplification, Sequencing, Fluorescence
Journal: bioRxiv
Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy
doi: 10.64898/2026.04.06.716727
Figure Lengend Snippet: Sorting scheme to enrich astrocytes, oligodendrocytes, Prox1-negative neurons and Prox1-positive neurons for amplicon sequencing.
Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X),
Techniques: Amplification, Sequencing
Journal: Angiogenesis
Article Title: A xenograft model for venous malformation
doi: 10.1007/s10456-018-9624-7
Figure Lengend Snippet: Characterization of VM–EC morphology and endothelial marker expression. a VM–EC at 80–90% confluency stained positive for endothelial markers CD31, vWF, VE-Cadherin, and negative for lymphatic marker Prox1 and smooth muscle marker αSMA, similar to b control EC (cord blood endothelial colony forming cells, cbEFCF; human umbilical vein endothelial cells, HUVEC; Foreskin EC). Foreskin EC were a positive control for Prox1 staining, and CD31 − cells (non-EC isolated from VM1 tissue) were a positive control for αSMA. Specific markers (green), nuclei (blue). Scale bars: phase 100 µm; immunofluorescence 50 µm. c qRT-PCR of CD31, VWF, and VE-Cadherin gene expression of VM–EC and cbECFC, normalized to HUVEC. n = 3 independent repeats, in triplicate
Article Snippet: Primary antibody incubation with anti-CD31 (1:50, Dako), vonWillebrand Factor (vWF) (1:100, Dako), αSMA (1:500, Sigma), VE-Cadherin (1:50, Santa Cruz), and
Techniques: Marker, Expressing, Staining, Positive Control, Isolation, Immunofluorescence, Quantitative RT-PCR